|Speciale, 3. modul, 2011, id:275|
|Vejleder:||Dorthe Posselt og Jens Spanget-Larsen|
|Findes på RUb:||Ja|
The aim of this thesis is to aid in the understanding of the thylakoid. Thylakoid or thylakoid membrane is a term coined by W. Mente i 1960 for the internal chloroplast membrane system. The thylakoid is a complex membrane system divided into circular membrane stacks, called grana lamella, connected by fretlike membranes called stroma lamella. This is approached by investigation the thylakoid with 3 different methods; small angle x-ray scattering, transmussion electron microscopy, and spectroscopy. The investigated thylakoid systems are isolated from Pisum sativum and Arabidopsis thaliana commonly known as peas and blue in order to influence different parts of the thylakoid structure. The arabidopsis was mutated to inhibit the synthethis of a primary protein, which should show a major influence on the thylakoid system. The study focuses on the contribution of each experimental approach to improve the interpretation of the SAXS data through a model system. The model system choosen in the study is a model developed by Jens K. Holm to interpret thylakoid membrane flexability and changes from physico-chemical environmental change such as light induced effects and osmolarity. The model work well for the unchanged system without mutations or artificial light but struggles to interpret SAXS data obtained from thylakoid with artificial growth conditions.