|Internt-fagligt, 2 + 3. modul, 2007/2008, id:248|
|Vejleder:||Tage E. Christensen|
|Findes på RUb:||Ja|
In this project we research, whether or not a planar carbon film and the 3omega-method can be used to measure the denaturational enthalpy of lysozyme. During the course of this work we encountered unforseen problems related to the usage of carbon films in the 3omega-method. It is shown that the gold-carbon junction of certain films to some estenc behaves like a Schottky Diode. Furthermore an annealing-like process is observed, in which the resistance of the carbon films declines at 330K. The effusivity of glycerol is found within a 14% accuracy. This is in all probability is not accurate enough to observe the denaturational enthalpy of lysozyme. The following conclusions are drawn: 1) Carbon films and the 3omega-method cannot at the present time be used to measure the denaturational enthalpy of lysozyme, 2) If however the problems encounted in this project are worked through the 3omega detection technique might eventually be applicable to proteins.